Ham’s F12 Medium, w: 1.0 mM stable Glutamine, w: 1.0 mM Sodium pyruvate, w: 1.176 g/L NaHCO3
Ham’s F-12 Medium, also known as Ham’s F-12 Nutrient Mix, is a widely used basal medium designed specifically for cell culture. It has been extensively employed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells, lung cells, and mouse L cells. Additionally, Ham’s F-12 is the medium of choice for a Clonal Toxicity Assay (CTA). One of its notable advantages is the ability to support cell growth without the need for serum supplementation. This eliminat…
Ham’s F12 Medium, w: 1.0 mM stable Glutamine, w: 1.0 mM Sodium pyruvate, w: 1.176 g/L NaHCO3
Ham’s F-12 Medium, also known as Ham’s F-12 Nutrient Mix, is a widely used basal medium designed specifically for cell culture. It has been extensively employed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells, lung cells, and mouse L cells. Additionally, Ham’s F-12 is the medium of choice for a Clonal Toxicity Assay (CTA). One of its notable advantages is the ability to support cell growth without the need for serum supplementation. This eliminates potential interference caused by serum components, ensuring consistent and reliable experimental results. By providing a serum-free culture environment, Ham’s F-12 Medium offers researchers greater control over their investigations. Another key feature of Ham’s F-12 Medium is its suitability for single-cell plating. This makes it an excellent choice for a variety of cell lines, including CHO cells, lung cells, and mouse L cells. The medium’s optimized nutrient composition facilitates efficient attachment and growth of individual cells, enabling the establishment of homogeneous cell cultures with improved reproducibility. Moreover, Ham’s F-12 Medium has gained recognition as the preferred medium for the Clonal Toxicity Assay (CTA). This assay plays a critical role in assessing the cytotoxic effects of substances on cells. By utilizing Ham’s F-12 Medium in the CTA, researchers can accurately evaluate the impact of various compounds or treatments on individual cells, providing valuable insights into toxicological profiles. Quality control
pH = 7.2 +/
- 0.02 at 20-25°C. Each lot has been tested for sterility and absence of mycoplasma and bacteria.
Maintenance
Keep refrigerated at +2°C to +8°C in the dark. Freezing and warming up to +37° C minimize the quality of the product. Do not heat the medium to more than 37° C or use uncontrollable sources of heat (e.g., microwave appliances). If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature. Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture.
Composition
Components mg/L
Inorganic Salts Calcium chloride x 2H2O 44,00
Copper(II) sulfate x 5H2O 0,00
Iron (II) sulfate x 7H2O 0,83
Magnesium chloride x 6H2O 122,00
Potassium chloride 223,65
Sodium chloride 7599,00
di-Sodium hydrogen phosphateanhydrous 142,04
Zinc sulfate x 7H2O 0,86
Other Components D(+)-Glucose anhydrous 1801,60
Hypoxanthine 4,08
Linoleic acid 0,08
DL-α-Lipoic acid 0,21
Phenol red 1,20
Putrescine x 2HCl 0,16
Sodium pyruvate 110,00
Thymidine 0,73
NaHCO3 1176,00
Amino Acids L-Alanine 8,91
L-Arginine x HCl 210,70
L-Asparagine x H2O 15,01
L-Aspartic acid 13,31
L-Cysteine x HCl x H2O 35,12
L-Alanyl-L-Glutamine 217,30
L-Glutamic acid 14,71
Glycine 7,51
L-Histidine x HCl x H2O 20,96
L-Isoleucine 3,94
L-Leucine 13,12
L-Lysine x HCl 36,54
L-Methionine 4,48
L-Phenylalanine 4,96
L-Proline 34,53
L-Serine 10,51
L-Threonine 11,91
L-Tryptophan 2,04
L-Tyrosine 5,44
L-Valine 11,71
Vitamins D(+)-Biotin 0,01
D-Calcium pantothenate 0,24
Choline chloride 13,96
Folic acid 1,32
myo-Inositol 18,02
Nicotinamide 0,04
Pyridoxine x HCl 0,06
Riboflavin 0,04
Thiamine x HCl 0,34
Vitamin B12 1,36
€25.00*
Variants: 100 ml
Accutase Cell Dissociation Reagent
- A Gentle Alternative to Trypsin Accutase is a cell detachment solution that is revolutionizing the cell culture industry. It is a mix of proteolytic and collagenolytic enzymes that mimics the action of trypsin and collagenase. Unlike trypsin, Accutase does not contain any mammalian or bacterial components and is much gentler on cells, making it an ideal solution for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware. In this blog post, we will explore the benefits and uses of Accutase and how it is changing the game in cell culture. Advantages of Accutase Accutase has several advantages over traditional trypsin solutions. Firstly, it can be used whenever gentle and efficient detachment of any adherent cell line is needed, making it a direct replacement for trypsin. Secondly, Accutase works extremely well on embryonic and neuronal stem cells, and it has been shown to maintain the viability of these cells after passaging. Thirdly, Accutase preserves most epitopes for subsequent flow cytometry analysis, making it ideal for cell surface marker analysis. Additionally, Accutase does not need to be neutralized when passaging adherent cells. The addition of more media after the cells are split dilutes Accutase so it is no longer able to detach cells. This eliminates the need for an inactivation step and saves time for cell culture technicians. Finally, Accutase does not need to be aliquoted, and a bottle is stable in the refrigerator for 2 months. Applications of Accutase Accutase is a direct replacement for trypsin solution and can be used for the passaging of cell lines. Additionally, Accutase performs well when detaching cells for the analysis of many cell surface markers using flow cytometry and for cell sorting. Other downstream applications of Accutase treatment include analysis of cell surface markers, virus growth assay, cell proliferation, tumor cell migration assays, routine cell passage, production scale-up (bioreactor), and flow cytometry. Composition of Accutase Accutase contains no mammalian or bacterial components and is a natural enzyme mixture with proteolytic and collagenolytic enzyme activity. It is formulated at a much lower concentration than trypsin and collagenase, making it less toxic and gentler, but just as effective. Efficiency of Accutase Accutase has been shown to be efficient in detaching primary and stem cells and maintaining high cell viability compared to animal origin enzymes such as trypsin. 100% of cells are recovered after 10 minutes, and there is no harm in leaving cells in Accutase for up to 45 minutes, thanks to autodigestion of Accutase. In summary In conclusion, Accutase is a powerful solution that is changing the game in cell culture. With its gentle nature, efficiency, and versatility, Accutase is the ideal alternative to trypsin. If you are looking for a reliable and efficient solution for cell detachment, Accutase is the solution for you.
€75.00*
Phosphate-Buffered Saline (PBS) Solution Phosphate-buffered saline (PBS) is a widely used buffer solution in biological and chemical research. It plays a crucial role in maintaining the pH balance and osmolarity during various experimental procedures, including tissue processing and cell culture. Our PBS solution is meticulously formulated with high-purity ingredients to ensure stability and reliability in every experiment. The osmolarity and ion concentrations of our PBS closely mimic those of the human body, making it isotonic and non-toxic to most cells.
Composition of Our PBS Solution Our PBS solution is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions. At a 1X working concentration, it contains:
8000 mg/L Sodium chloride (NaCl) 200 mg/L Potassium chloride (KCl) 1150 mg/L Sodium phosphate dibasic anhydrous (Na2HPO4) 200 mg/L Potassium phosphate monobasic anhydrous (KH2PO4)
This composition ensures an optimal pH and ionic balance, suitable for a wide range of biological applications.
Applications of Our PBS Solution Our PBS solution is ideal for various applications in biological research. Its isotonic and non-toxic properties make it suitable for substance dilution and cell container rinsing. PBS solutions containing EDTA are effective for disengaging attached and clumped cells. However, divalent metals such as zinc should not be added to PBS, as this can cause precipitation. In such cases, Good’s buffers are recommended. Additionally, our PBS solution is an acceptable alternative to viral transport medium for the transport and storage of RNA viruses, including SARS-CoV-2.
Quality Control
Sterile-filtered
Storage and Shelf Life
Store at +2°C to +25°C, protected from light. Once opened, store at 2°C to 25°C and use within 24 months.
Shipping Conditions
Ambient temperature
Maintenance
Keep refrigerated at +2°C to +8°C in the dark. Avoid freezing and frequent warming to +37°C, as it reduces product quality. Do not heat the medium beyond 37°C or use uncontrolled heat sources such as microwave appliances. If only part of the medium is to be used, remove the required amount and warm it to room temperature before use.
Composition
Category Components Concentration (mg/L)
Salts Potassium chloride 200
Potassium phosphate monobasic anhydrous 200
Sodium chloride 8000
Sodium phosphate dibasic anhydrous 1150
€20.00*