Membrane Kymograph Generator: A cross-platform GUI software for automated generation and analysis of kymographs along dynamic cell boundaries (opens in new tab)

AbstractSummaryThe plasma membrane and accompanying cortex serve as major hubs of signal transduction and cytoskeletal activities that collectively regulate cell physiological processes such as migration, polarity, macropinocytosis, phagocytosis, and cytokinesis. Yet, dynamically tracking membrane-cortex associated protein or lipid kinetics from live-cell image series remains challenging, primarily due to the difficulty of accurately extracting and aligning the cell boundary between consecuti...