Harnessing the murine inner cell mass mechanical environment enhances derivation of in vitro nascent primitive endoderm precursor cells (opens in new tab)

ABSTRACTStem cell-based models resembling murine blastocysts represent a useful system to investigate subsequent developmental processes. While existing cell lines derived from epiblast and trophectoderm can be aggregated to form ‘blastoids’, some previously tested in vitro cultured extra-embryonic endoderm cells tended to progress to later stages of development, so integrated inefficiently into blastoids. We attempted to capture the precursor population for extra-embryonic endoderm in vitro ...