Beyond the canonical: The role of post-transcriptional regulation in drug-target interaction prediction (opens in new tab)

Author summary Computational drug–target interaction models have become central tools in modern drug discovery, yet they typically represent each gene with a single canonical protein sequence, overlooking the fact that most human genes produce multiple isoforms through alternative splicing. These isoforms can differ in structure and drug-binding properties, raising the question of whether current prediction models are robust to such biologically realistic variation. Here, we systematically ev...