HMCVelo: A Deterministic Model for Hydroxymethylation Velocity in Single Cells (opens in new tab)

I present hydroxymethylation velocity (HMCVelo), the first velocity framework for DNA methylation dynamics. HMCVelo is a deterministic ordinary differential equation (ODE) model that computes the time derivative of hydroxymethylation state for individual cells and genes. The model exploits a recent advance in single-cell epigenomics - Joint single-nucleus hydroxymethylcytosine and methylcytosine sequencing (Joint-snhmC-seq) - which enables subtraction-free quantification of 5-hydroxymethylcyt...