AssayBLAST v2: Major update improving reliability and reporting of the in silico analysis of molecular multi-parameter assays (opens in new tab)

Introduction: Accurate in silico evaluation of primers and probes is essential for the rational design of molecular multi-parameter assays. We present AssayBLAST v2 to automate and simplify this process for extensive assay designs. Results: A newly integrated strand and proximity check enables precise validation of corresponding oligonucleotides, ensuring correct orientation and spacing for efficient amplification. Based on predicted oligonucleotide interactions, AssayBLAST v2 estimates ampli...