NativeReady: an open benchmark and sequence-based triage model for native mass spectrometry suitability (opens in new tab)

Native mass spectrometry is a central analytical method for characterizing intact proteins, antibody-drug conjugates, and non-covalent assemblies, and it is increasingly the deciding measurement in biotherapeutic development pipelines. A single screening attempt requires days of expression, purification, and buffer exchange into ammonium acetate, followed by 30 to 60 minutes of optimization on a Q-Exactive UHMR or comparable instrument. To our knowledge, no published sequence-based predictor ...