Engineering a pacemaker-driven human mini-heart guided by spatial multi-omics of sinoatrial node development (opens in new tab)

The human sinoatrial node (SAN) functions as the primary pacemaker of the heart and coordinates the hierarchical electrical activity that drives cardiac contraction. However, experimental systems capable of reconstructing pacemaker driven cardiac organization in human tissues remain limited. Here we integrate spatial multi-omics of the human fetal SAN with stem cell engineering to generate pacemaker organoids (Sinoids) and assemble them into a pacemaker driven human mini-heart composed of sin...