ProCAST: A Bioinformatics Suite for Mass Spectrometry-Based Protein Corona Proteomics Analysis (opens in new tab)

When nanoparticles are exposed to biological fluids, they spontaneously adsorb proteins, forming a protein corona that defines their biological identity and dictates cellular uptake, biodistribution, and toxicity. Characterizing protein coronas includes using proteomics approaches (e.g., LC-MS/MS) to identify proteins and generate vast lists of adsorbed proteins, often visualized via complex heatmaps. While heatmaps display data they do not offer heuristic guide, leaving the driving mechanism...