DeSpotX: Identifiability-Based Decontamination for Spatial Transcriptomics (opens in new tab)

Spatial transcriptomics (ST) at single-cell resolution profiles gene expression in its native spatial context, but a substantial fraction of transcripts contaminate neighboring cells, compromising downstream biological analyses. Existing decontamination methods rely on heuristic priors and either ignore the spatial structure of contamination or aggregate over neighbors without separating contamination from native expression, leaving the decomposition ambiguous. To resolve this ambiguity, we i...