simCRISPR: Modeling experimental complexity in pooled CRISPR screens (opens in new tab)

Pooled CRISPR screens are widely used to investigate gene function and uncover genetic interactions. However, benchmarking computational methods for detecting gene-by-environment (GxE) interactions remains difficult because ground truth is rarely available and existing simulation tools are not designed for GxE screening contexts. To address this, we developed texttt{simCRISPR}, a flexible simulation framework for generating pooled CRISPR screen data under complex experimental designs. Using s...