Multi-layer transcriptomic characterization of age-related immune dynamics (opens in new tab)

Despite the pivotal role of mRNA isoform diversity in governing immune cell function, current investigations into peripheral immune aging predominantly focused on gene-level expression, obscuring deeper regulatory layers of transcriptome complexity. Here, we leveraged a 5' scRNA-seq atlas comprising approximately 2.5 million PBMCs from 378 healthy donors. We demonstrate that immune aging is characterized by profound, non-linear transcriptional reprogramming that extends beyond gene-level shif...