Reproducible transcriptional modules define glioblastoma ecosystems across independent cohorts. (opens in new tab)

Glioblastoma (GBM) comprises a complex ecosystem of malignant, immune, vascular and neural transcriptional states. However, it remains difficult to determine which gene expression programmes are reproducibly recovered across independent cohorts and profiling platforms, because programme-level analyses are sensitive to cohort composition, technical context and factorization rank. Here, we analyzed three public GBM datasets--GLASS and IVYGAP bulk RNA-seq cohorts and the HEILAND Visium spatial t...