L3R-seq: A long-read 3'RACE approach for deep quantitative analysis of RNA processing (opens in new tab)

Long-read sequencing technologies offer the potential to capture multiple RNA processing events within single molecules, but standard protocols suffer from quantification biases and sequencing errors that limit their utility for precise analysis. Here, we describe Long-read 3' RACE-seq (L3R-seq), a targeted long-read sequencing method that ligates a unique molecular identifier (UMI)-containing adapter to the 3' end of RNA molecules prior to reverse transcription and PCR amplification. By grou...