Prediction and evaluation of Split-ORFs using Ribo-seq data (opens in new tab)

Split Open Reading frames (Split-ORFs) occur in transcripts containing at least two open reading frames, each encoding a part of the same full-length protein. These multiple open reading frames arise from alternatively spliced transcript isoforms. Split-ORFs have been described in the SR protein family of splicing factors, where the resulting protein halves play important autoregulatory roles. Here, we present the Split-ORF pipeline, a computational tool that predicts Split-ORFs from transcri...