Benchmarking normalisation methods for differential binding analysis in CUT&RUN (opens in new tab)

CUT&RUN (Cleavage Under Targets and Release Using Nuclease) is an increasingly popular method for profiling protein interactions (transcription factors, histone modifications, etc) with DNA across the whole genome. When performing differential binding analysis of CUT&RUN data to identify genomic regions where interaction profiles vary between conditions, data normalisation is essential for accurate biological interpretations. Despite this, there are no clear guidelines on the optimal normalis...