CryoARC: Atomic-resolution conformational landscapes of protein assemblies from cryo-EM single particles with evolutionary priors (opens in new tab)

Single-particle cryo-electron microscopy (cryo-EM) reveals structural heterogeneity in macromolecular complexes, but recovering continuous conformational landscapes at high resolution remains challenging. Here, we introduce CryoARC, a deep learning framework that integrates evolutionary sequence representations with cryo-EM particle images to reconstruct continuous conformational ensembles at atomic resolution. CryoARC combines a latent representation of particle heterogeneity with a sequence...