Systematic In Silico Off-Target Assessment of siRNAs: Integrated Tissue-Specific Scoring and Cross-Species Preclinical Model Selection with TargetSureR (opens in new tab)

Small interfering RNAs (siRNAs) have become a transformative class of nucleic acid therapeutics for clinical disease treatment, yet sequence-dependent off-target silencing continues to pose a major safety barrier that hinders their preclinical refinement and large-scale translational application. Existing bioinformatics tools only support partial off-target evaluation, either focusing on basic sequence optimization or simple seed-region scanning, and fail to deliver systematic, multi-dimensio...