Morphology-robust quantification of subcellular organization in complex cells (opens in new tab)

Quantitative analysis of subcellular protein organization is often confounded by variation in cell morphology, limiting the identification and interpretation of localization patterns in fluorescence microscopy data from morphologically complex cells, such as neurons and glia. We introduce CellAligner, an unsupervised framework that uses fused unbalanced Gromov-Wasserstein couplings to map protein distributions from morphologically distinct cells into shared anchor-cell geometries, enabling mo...