Development of the Mitochondrial Base Editor Analysis Package (MitoBEAP). (opens in new tab)

For many years, the genetic manipulation of mitochondrial DNA was largely hampered by inefficient delivery of nucleic acids to mitochondria. However, the development of mitoCBEs, such as mitochondrial cytosine base editors (DdCBEs), which catalyse C-to-T and G-to-A conversions, and more recently, mitoABEs, such as transcription-activator-like effector (TALE)-linked deaminases (TALEDs) enabling A-to-G and T-to-C conversion, has transformed this field. Generally, mitochondrial base editors exhi...