Single-Cell Multi-Omics Dissection of Malignant Evolutionary Mechanisms and Construction of a Prognostic Model for Clear Cell Renal Cell Carcinoma (opens in new tab)

Clear cell renal cell carcinoma (ccRCC) exhibits pronounced heterogeneity across WHO histological grades, yet systematic single-cell multi-omics studies characterizing these transitions remain limited. We integrated scRNA-seq and scATAC-seq data across ccRCC WHO grades to establish a multi-omics framework encompassing tumor cells and immune populations. Using pseudotime trajectory analysis and machine learning ensembles, we developed a prognostic signature (CBG) from core nodes of transcripti...