Expert-Guided Supervised Annotation of Erythroid Differentiation in Single-Cell RNA-seq (opens in new tab)

Accurate annotation of intermediate cell states remains a major challenge in single-cell RNA sequencing (scRNA-seq), particularly in continuous differentiation systems such as erythropoiesis. Existing reference-based methods often lack the resolution required to distinguish early and transitional erythroid progenitors and may generalise poorly across datasets and modalities. Here, we present a supervised framework for erythroid lineage annotation based on expert-curated training data that int...