Deep coverage and extended sequence reads obtained with a single archaeal protease expedite de novo protein sequencing by mass spectrometry (opens in new tab)

Highly efficient hyperthermal acidic proteases combined with hybrid-fragmentation schemes provide five times more unique peptide reads than trypsin or chymotrypsin, greatly boosting confidence in de novo sequencing. This approach enables confident de novo antibody sequencing based on a single LC-MS run.