From 5F hematopoietic progenitors to osteoclasts: a scalable human model of osteoclastogenesis (opens in new tab)

IntroductionHuman osteoclast models are essential for studying bone physiology and skeletal disease, yet existing systems are constrained by donor variability and the limited expansion capacity of hematopoietic progenitors.MethodsConditionally expandable 5F hematopoietic stem and progenitor cells generated by transcription factor reprogramming with HOXA9, ERG, RORA, SOX4, and MYB were differentiated into osteoclasts. Cells were driven through an intermediate monocyte stage using IL three and ...