Activation of mitochondrial CPS1 promotes dormant ovarian follicle activation via arginine elevation and the mTORC1 pathway (opens in new tab)

BackgroundCarbamoyl phosphate synthetase 1 (CPS1) is a mitochondrial ligase essential for urea production, catalyzing the formation of carbamoyl phosphate from ammonia in a reaction requiring the allosteric activator N acetyl L glutamate (NAG). Through the urea cycle, CPS1 activity ultimately contributes to arginine synthesis, a known activator of mTOR. The NAG analogue N-carbamoyl-L-glutamate (NCG) has been shown to promote follicle development in several species, potentially via mTOR signal...