Regulation of H-type angiogenesis and permeability in the subchondral bone of osteoarthritis: the role of Slit3 and the Robo4/Rac1-GTP/ROS axis (opens in new tab)

Subchondral bone H-type angiogenesis and increased permeability are critical in osteoarthritis (OA) progression, yet the underlying regulatory mechanisms remain unclear. This study utilized proteomic analysis to identify significantly elevated expression of Slit guidance ligand 3 (Slit3) and Ras-related C3 botulinum toxin substrate 1 (Rac1) in the subchondral bone of patients with OA, both associated with angiogenesis and permeability. Experimental validation revealed that Slit3 and Rac1-GTP,...