Phenotypic transformation of HCC827 cells revealed by evaluation of human platelet lysate as a sustainable fetal bovine serum replacement (opens in new tab)

Fetal bovine serum (FBS), a widely used supplement in cell culture, raises ethical and scientific concerns due to its animal origin, batch variability, and limited physiological relevance for human cells. As part of our efforts to adopt more sustainable and human-relevant cell culture conditions, we investigated human platelet lysate (HPL) as an alternative to FBS for culturing non-small cell lung carcinoma (NSCLC) cells. Different compositions of both sera were assessed by ELISA, showing com...