In vivo CRISPR-mediated activation of cardiogenic genes to reprogram cardiac fibroblasts (opens in new tab)

BackgroundWe previously demonstrated that fibroblasts can be reprogrammed through a proliferative progenitor stage to drive both cardiomyogenesis and neovascularization. However, it remains to be determined whether cardiac fibroblasts (CFs), the primary mediators of post-injury remodeling, retain the plasticity to be concurrently redirected into cardiovascular lineages within the in vivo environment.MethodsThis study aimed to activate endogenous cardiogenic genes (Gata4, Nkx2.5, Tbx5, Isl1, a...