Live monitoring uncovers divergent epigenetic remodeling during osteogenic and adipogenic differentiation of mesenchymal stem cells (opens in new tab)

Histone post-translational modifications represent a central mechanism of epigenetic regulation, upholding chromatin architecture and genomic function. Elucidating the dynamic relationship between epigenetic modifications and chromatin remodeling requires sensitive tools for live-cell analysis. To address this, we developed K9-MILo (K9-Methylated Inhibitory Locus), a genetically encoded fluorescent sensor for visualizing the heterochromatin mark H3K9me3 in living cells. K9-MILo incorporates t...