Serum withdrawal establishes a stress-dominant entry state during myogenic differentiation (opens in new tab)

IntroductionSkeletal myogenesis in C2C12 cultures is typically induced by switching confluent cells from high-serum growth medium to low-serum differentiation medium. Because serum withdrawal is itself an acute physiological perturbation, early post-switch transcriptional changes may reflect varying mixtures of lineage progression and stress adaptation.MethodsHere we performed a descriptive reanalysis of publicly available bulk RNA-seq datasets spanning (i) canonical C2C12 differentiation by ...