Proteomic and transcriptomic signatures of cytoskeletal remodeling during morphogenesis in the basal metazoan Halisarca dujardinii (Porifera) (opens in new tab)

Sponges are capable of rebuilding entire functional organisms from dissociated somatic cells. This feat demands radical cytoskeletal reprogramming whose molecular logic remains obscure. Using the Arctic demosponge Halisarca dujardinii, we mapped molecular signatures across free-swimming larvae, sessile adults, and early cell aggregates. Integrating RNA-seq, single-cell deconvolution, DIA-LC-MS proteomics, native complex fractionation, live-cell proteasome imaging, and immunofluorescence, we i...