EphA2 sustains the adaptive response of colorectal organoids to chemotherapy (opens in new tab)

IntroductionEphA2 is highly expressed in colorectal cancer (CRC), and high EphA2 expression indicates a worse prognosis. We investigated EphA2 dynamics in a clinically relevant model: CRC patient-derived organoids (PDOs) treated with chemotherapy.MethodsWe evaluated the number of EphA2-expressing cells and the Aldehyde dehydrogenase activity by flow cytometry, and analyzed EphA2 protein levels and phosphorylation status using Zn-Phos-tag gels and indirect ELISA. We employed siRNA to deplete t...