Novel feeder-cell-free 3C system promotes the transition from primed to formative pluripotency, self-renewal, and germline differentiation in rabbit embryonic stem cells in vitro (opens in new tab)

IntroductionMost rabbit embryonic stem (rbES) cell lines established in embryonic fibroblast feeder layer systems predominantly exhibit pluripotency features restricted to a “primed” state, which hinders their directed differentiation into reproductive germ cells and limits their capacity for germline transmission.MethodsWe developed a novel 3C system containing IWP2, DZNep, and TSA in iFLY medium supplemented with basic fibroblast growth factor, human leukemia inhibitory factor, and Noggin. ...