Defining neurovascular ecosystem states with single-cell multi-omics and machine learning: insights into cerebrovascular remodeling in the tumor context (opens in new tab)

ObjectiveTo define neurovascular ecosystem states within the tumor microenvironment and elucidate the regulatory basis of cerebrovascular remodeling through single-cell multi-omics and interpretable machine learning.MethodsAn orthotopic murine brain tumor model was established. Single-cell RNA sequencing (scRNA-seq), single-cell assay for transposase-accessible chromatin sequencing (scATAC-seq), regional tissue validation, and integrative computational analyses were performed to characterize ...