Palmitoyltransferase DHHC7 mediates protein palmitoylation and is essential for sperm function through the modulation of [Ca2+]i and ROS signaling (opens in new tab)

BackgroundPalmitoylacyltransferase DHHC7 (DHHC7) plays a critical role in various biological processes and diseases. However, its expression, localization, and regulatory mechanisms in sperm function remain largely unknown.MethodsImmunofluorescent staining was used to localize DHHC7 in sperm and examine its colocalization with the estrogen receptor (ER), progesterone receptor (PR), and caveolin-1 (CAV1). To explore DHHC7’s role in sperm function, sperm were treated with a specific DHHC7 antib...