Multiscale imaging of polarity in bovine oviductal organoids (opens in new tab)

Organoids are three-dimensional cell culture systems that recapitulate key structural features of their tissue of origin; however, interpretation of organoid architecture is inherently dependent on the imaging modality used. In organoids cultured within extracellular matrix (ECM), cells typically adopt an apical-in/basal-out configuration in which the apical surface faces an enclosed lumen, limiting direct visualization and access to the luminal surface. Experimental manipulation of polarity ...