MBNL1-dependent alternative splicing promotes neuronal differentiation through regulation of NUMA1 exon 16 during fibroblast-to-neuron reprogramming (opens in new tab)

IntroductionDirect neuronal reprogramming enables the generation of neurons from somatic cells without passing through a pluripotent state, yet the post-transcriptional mechanisms that refine neuronal identity after fate induction remain poorly understood.MethodsWe examined alternative splicing during fibroblast-to-neuron reprogramming and investigated the effects of MBNL1 knockdown on neuronal phenotype, transcriptomic and splicing changes, and NUMA1 exon 16 regulation.ResultsMBNL1 knockdown...