UNIQUE: ultrasound non-destructive in-situ quantitative evaluation of stem cell spheroid deformability during differentiation into specific lineages (opens in new tab)

Reliable assessment of 3D stem cell spheroid function and fate is crucial in regenerative medicine and cell therapy, where gene and protein analyses and mechanical probing are employed. However, current methods are often invasive and struggle with heterogeneity, frequently damaging spheroid integrity, which drives a need for non-destructive, label-free techniques to assess individual spheroids directly in their culture environment. Here, we present a label-free platform, UNIQUE (Ultrasound No...