pH-responsive amphiphilic peptide nanofibers facilitate endosomal escape for cytosolic protein delivery (opens in new tab)

The therapeutic use of proteins often depends on cytosolic access, which is frequently limited by endosomal trapping. Here, we developed pH-responsive amphiphilic peptide nanofibers (NFs) that may facilitate cytosolic protein delivery by selectively destabilizing endosomal membranes. We prepared a series of NFs by varying the number of glutamic acid residues (CE1, CE2, and CE3) appended to β-sheet-forming peptides. The resulting nanofibers were uniform in width and presented hydrophobic surfa...