The genetic architecture of an allosteric hormone receptor (opens in new tab)

Many proteins function as switches, transducing the concentrations of environmental chemicals into cellular responses. It is not well understood how signal processing by switches is genetically encoded. Here, using a massively parallel approach, GluePCA, we present >40,000 measurements and a complete map of how mutations alter the quantitative activation function of a receptor switch, the plant hormone sensor PYL1. Close to 90% of missense variants tune the dose-response of the receptor, ofte...