Burden-aware feedback control of microbial consortia (opens in new tab)

Engineered microbial consortia are emerging as programmable systems capable of sensing and responding to their environment. However, maintaining defined community composition over time remains challenging, particularly in bioprocesses where growth conditions and metabolic burdens continuously shift. Here, we develop a burden-aware multicellular RNA-based feedback control system that stabilises coculture composition by coupling gene expression burden to growth regulation. The system integrates...