Engineering B cells to express fully customizable antibodies with enhanced Fc functions (opens in new tab)

Genome editing within the constant region of the immunoglobulin Heavy chain locus (IGH) can reprogram B cells to express heavy-chain-only antibodies (HCAbs) containing custom antigen-recognition domains. HCAb-engineered cells express both surface B cell receptor (BCR) and secreted antibody isoforms and respond to antigen. Here, we extend this approach to also allow customization of the constant (Fc) domain of the Heavy chain by selecting alternate editing sites within IGH, producing HCAbs wit...