Parallel enzymatic DNA synthesis using a semiconductor chip (opens in new tab)

Parallelized DNA synthesis across a dense array of sites is crucial to high-throughput synthetic biology and diagnostics and could potentially be used for DNA-based data storage. Phosphoramidite synthesis can achieve substantial parallelism but relies on harmful solvents and centralized facilities. Enzymatic DNA synthesis in mild aqueous solution is safer and could be more accessible, but parallel demonstrations remain modest at an early stage. Here we show that a complementary metal–oxide–se...