Proteomic maps of synapses and synaptic vesicles across the central and enteric nervous systems (opens in new tab)

While the synaptic proteome of the central nervous system (CNS) has been extensively characterized, its composition in the peripheral nervous system (PNS) remains unknown. There is an urgent need to delineate the precise proteomic landscape of the enteric nervous system (ENS), often referred to as the “second brain,” and to systematically compare its molecular heterogeneity with that of the CNS. To address this, we developed a streamlined workflow integrating subcellular organelle isolation w...