Constructing artificial methylotrophic S. cerevisiae for chondroitin sulfate production using a multidimensional combinatorial strategy (opens in new tab)

The concept of a methanol-based circular bioeconomy envisions the conversion of greenhouse gases into one-carbon compounds, which are subsequently utilized to produce high-value-added products. However, the toxicity of intermediates limits the metabolic efficiency within chassis strains. Here a carbon metabolism reconfiguration strategy combined with adaptive laboratory evolution was applied to engineer an artificial methylotrophic yeast by accelerating the flux of toxic intermediates into th...