Membrane remodelling for increased methanol tolerance in Komagataella phaffii (opens in new tab)

Methanol is a promising C1 feedstock for bioproduction but its cytotoxicity limits applications. Here we enhanced Komagataella phaffii GS115 methanol tolerance to 10–13% (v/v) by overexpressing four genes (phosphatidylethanolamine methyltransferase (PET2), phosphatidylserine synthase (PSA), PAS_chr1-4_0178 (0178) and PAS_chr2-2_0267 (0267)) (S33 strain). Our results show that, under high methanol stress, S33 undergoes membrane lipid remodelling-driven metabolic reprogramming, with central car...